H.įabrication of biocompatible microparticles, such as alginate particles, with the possibility of controlling the particles' morphology in a high-throughput manner, is essential for pharmaceutical and cosmetic industries. Jeyhani, Morteza Mak, Sze Yi Sammut, Stephen Shum, Ho Cheung Hwang, Dae Kun Tsai, Scott S.
However, further works are required to assure maintaining the activity of the enzyme/protein after electrospray.Ĭontrolled electrosprayed formation of non-spherical microparticles Conclusion: Our study showed that electrospraying could be an interesting approach to encapsulate proteins/enzymes in polymeric nanoparticles. Activity of the enzyme was also checked 30 min after preparation and 19.2% activity was shown. Nearly, 20% of SK was released in the first 30 minutes, followed by cumulative release of 41% during 72 h. The optimum preparation had mean (SD size, encapsulation efficiency and loading capacity of 37 (12 nm, 90% and 8.2%, respectively. Also, minimum SD was obtainable at high values of voltage. Results: From the results, to obtain lowest size of nanoparticles, ratio of polymer/enzyme and needle internal diameter (ID should be low. Optimizing the SD has been rarely reported so far in electrospray. Effect of three input parameters, namely, voltage, internal diameter of needle (nozzle and concentration ratio of polymer to protein on size and size distribution (SD of NPs was evaluated using artificial neural networks (ANNs. This is the first paper which investigates activity of an electrosprayed enzyme. Methods: In this study, we used electrospray to encapsulate SK in PLGA NPs and evaluate its activity.
Streptokinase (SK is an important medicine for thrombotic diseases. Preparation, Optimization and Activity Evaluation of PLGA/Streptokinase Nanoparticles Using Electrosprayĭirectory of Open Access Journals (Sweden)įull Text Available Purpose: PLGA nanoparticles (NPs have been extensively investigated as carriers of different drug molecules to enhance their therapeutic effects or preserve them from the aqueous environment. The pulse duration of the energized voltage can be less than 1 millisecond and the frequency of the pulse wave can range from 30 to 800 Hz. The relaxation duration of the relaxation voltage can range from 1 millisecond to 35 milliseconds. The power system can supply voltage to the working electrode in the form of a pulse wave, where the pulse wave oscillates between at least an energized voltage and a relaxation voltage. The counter electrode can be proximate to, but separated from, the spray tip. The emitter can include a liquid conduit, a primary working electrode having a liquid contacting surface, and a spray tip, where the liquid conduit and the working electrode are in liquid communication. The electrospray ion source can include an emitter, a counter electrode, and a power supply. Kertesz, Vilmos [Knoxville, TN Van Berkel, Gary [Clinton, TNĪn electrospray ion source and method of operation includes the application of pulsed voltage to prevent electrolysis of analytes with a low electrochemical potential. Pulsed voltage electrospray ion source and method for preventing analyte electrolysis
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